I don't remember seeing an option for anisotropic filtering in the game options, but it does have AA there.
Personally I set my aniso filtering at the driver level, using ATI tray tools. I have a custom profile for Guild Wars that loads up with AA set on "application preference" and Aniso to 16x and Quality filtering (may be overkill there but I still get fantastic performance).
And believe me, I can see the difference when it's not on. The place to see the difference the most is on the ground, stretching out in front of you. Without Aniso filtering you will see "blurr bars" ahead of you, and especially on terrain that is more detailed like bricks. It's not as easy to notice at all if you are walking in the desert for example. The terrain is not detailed enough. Places like LA (bricky paved areas), and the parched/cracked ground in Ascalon are places you can see the difference more.
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